Journal: Biology

Article Title: The Capacity of Magnesium to Induce Osteoclast Differentiation Is Greatly Enhanced by the Presence of Zoledronate

doi: 10.3390/biology12101297

Figure Lengend Snippet: Flow cytometry analysis of the CD11b myeloid differentiation marker performed upon treatment with MgCl 2 , ZA, or both in osteoclasts derived from U937 cells by stimulation with PMA and VD3. Expression of the CD11b surface antigen was assessed by flow cytometry. The ( upper panel ) is a histogram showing, on the y axis, the mean fluorescent intensity (MFI) measured upon staining with an anti-CD11b PE antibody and indicated as mean ± S.E.M. The x axis shows the different treatments received by osteoclasts derived from U937 cells upon stimulation with PMA and VD3: None (Ctr), MgCl 2 (Mg), Zoledronate (ZA) or both (ZA + Mg). The ( lower panel ) is a flow cytometry histogram of a representative experiment, in which the y axis shows the percentage of cells, while the x axis shows the MFI. The treatments are highlighted by different colors. Ctr- refers to undifferentiated U937 cells, that were not stimulated with PMA and VD3, while Ctr+ are differentiated U937 cells that were stimulated with PMA and VD3 but did not receive Mg or ZA treatment.

Article Snippet: To this aim, flow cytometry was preceded by an incubation of samples for a 30 min at 4 °C with phycoerythrin-conjugated (PE) mouse anti-human CD11b Monoclonal Antibody (MoAb-) (Miltenyi Biotec, Auburn, CA, USA), in PBS containing 5% FCS and 1% FcR blocking reagent (Miltenyi).

Techniques: Flow Cytometry, Marker, Derivative Assay, Expressing, Staining

Journal: iScience

Article Title: Characterizing infection of B cells with wild-type and vaccine strains of measles virus

doi: 10.1016/j.isci.2023.107721

Figure Lengend Snippet:

Article Snippet: mouse monoclonal phycoerythrin conjugated anti-human CD27 , BD PharmigenTM , Cat#555441; RRID: AB_395834.

Techniques: Virus, Staining, Cell Isolation, Control, Software